The binding of nucleic acid to magnetic beads mainly relies on electrostatic, hydrophobic and hydrogen bonding. The DNA/RNA in the cell or tissue is released under the action of the lysis solution. At this time, the surface-modified superparamagnetic silica nanomagnetic beads “specifically bind” with nucleic acid to form a “nucleic acid-magnetic bead complex”. Then under the action of an external magnetic field, the complex is separated. After the eluate is washed to remove the non-specifically adsorbed magazines, desalted, and purified, the nucleic acid substance to be extracted is obtained.
The nucleic acid extraction principle is according to surface of superparamagnetic nanoparticles is modified and modified by nanotechnology to prepare superparamagnetic silica nanomagnetic beads. The magnetic beads can specifically recognize and efficiently bind with nucleic acid molecules on the microscopic interface. Using the superparamagnetism of silica nanospheres, under the action of Chaotropic salts (guanidine hydrochloride, guanidine isothiocyanate, etc.) and an external magnetic field, DNA and DNA can be separated from samples such as blood, animal tissues, food, and pathogenic microorganisms. RNA can be used in clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbiological testing, food safety testing, molecular biology research and other fields.
In fact, since testing institutions need to process a large number of samples every day, most of the nucleic acid extraction steps are carried out by means of an automatic nucleic acid extraction instrument with a magnetic bead method nucleic acid extraction kit.